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817501 Supelco

TSKgel® Octadecyl-2PW HPLC Column

phase C18 (octadecyl), L × I.D. 15 cm × 6 mm, 5 μm particle size

Synonym: TSKgel® Reversed Phase HPLC Column (Polymer-based)

  •  eCl@ss 32110501

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Properties

Related Categories Analytical/Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, TSKgel HPLC Columns, TOYOPEARL Bulk Media & Accessories, TSKgel Octadecyl-2PW Columns (125 Å pores),
material   stainless steel hardware
mfr. no.   Tosoh, 17501
extent of labeling   1 meq/mL ligand density (estimate)
parameter   0-100% organic solvent
  1.5 mL/min flow rate
  120 bar max. pressure
  4-50 °C temperature
application(s)   HPLC: suitable
L × I.D.   15 cm × 6 mm
matrix   polymer particle platform
matrix active group   C18 (octadecyl) phase
particle size   5 μm
pore size   125 Å
operating pH   2 - 12
mode of chromatography   Reversed phase

Description

General description

Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
• Octadecyl-2PW particles have 125Å pores
• Octadecyl-4PW particles have 500Å pores
• Phenyl-5PW RP particles have 1000Å pores
• Octadecyl-NPR particles are nonporous

Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-2PW provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The 125Å pores allow for anaylsis of peptides up to 8,000 Da. Columns packed with larger particle sizes are available for semi-preparative scale separations.

Physical form

Shipped in 55% acetonitrile - 45% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Price and Availability


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Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

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817503 TSKgel® Octadecyl-2PW Guard Column, phase C18 (octadecyl), L × I.D. 1 cm × 6 mm, 5 μm particle size

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