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818343 Supelco

TSKgel® Alpha-6000 HPLC Column

phase hydroxylated methacrylate, L × I.D. 30 cm × 7.8 mm, 13 μm particle size

Synonym: TSKgel® Alpha Columns

  •  eCl@ss 32110501



Related Categories Analytical/Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, TSKgel Alpha Columns, TSKgel Gel Permeation Columns for Polar Organic-Soluble Polymers,
material   stainless steel hardware
mfr. no.   Tosoh, 18343
parameter   ≤0.5 M salt concentration
  0-100% polar organic solvent
  1 mL/min flow rate
  20 bar max. pressure
  80 °C temperature
application(s)   HPLC: suitable
L × I.D.   30 cm × 7.8 mm
matrix   polymer particle platform
matrix active group   hydroxylated methacrylate phase
particle size   13 μm
pore size   ND
operating pH   2 - 12
suitability   suitable for L38 per USP
  suitable for L39 per USP
mode of chromatography   Size exclusion (SEC)


General description

TSKgel Alpha columns are composed of spherical, hydrophilic methacrylate beads. Unlike TSKgel PW-type columns, which are stable at most to a 50% organic mixed with water, TSKgel Alpha-type columns are stable in a wide variety of organic solvents at concentrations up to 100%. The main application area for TSKgel Alpha columns is the analysis of polymers that are soluble in polar organic solvents. Examples include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in DMF with 10mmol/L DMF), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in HFIP. The mixed-bed column, TSKgel Alpha-M, has an extended linear calibration range, and is suitable for samples with a broad MW distribution as well as samples with unknown molecular weight.

TSKgel Alpha-6000 columns are packed with rigid, porous, polymer beads which show minimal shrinkage or swelling in different solvent systems. They are best used for the separation of polymers that are soluble in polar organic solvents such as methanol, ethanol, DMF, THF, HFIP, DMSO, 2-propanol, and acetonitrile.

Physical form

Shipped in water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Price and Availability

All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis

Protocols & Articles

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818339 TSKgel® Alpha-2500 HPLC Column, phase hydroxylated methacrylate, L × I.D. 30 cm × 7.8 mm, 7 μm particle size
818342 TSKgel® Alpha-5000 HPLC Column, phase hydroxylated methacrylate, L × I.D. 30 cm × 7.8 mm, 10 μm particle size
818344 TSKgel® Alpha-M HPLC Column, phase hydroxylated methacrylate, L × I.D. 30 cm × 7.8 mm, 13 μm particle size

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