• USA Home
  • 818760 - TSKgel® Ether-5PW HPLC Column

EMAIL THIS PAGE TO A FRIEND
818760 Supelco

TSKgel® Ether-5PW HPLC Column

phase ether, L × I.D. 7.5 cm × 2 mm, 10 μm particle size

Synonym: TSKgel® Ether-5PW HPLC Column

  •  eCl@ss 32110501

Purchase

Properties

Related Categories Analytical/Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, Hydrophobic Interaction (HIC) Columns, TSKgel Ether-5PW Column (1000 Å pore size),
material   stainless steel hardware
mfr. no.   Tosoh, 18760
parameter   ≤4 M salt concentration
  ≤50% organic solvent
  0.12 mL/min flow rate
  10-50 °C temperature
  6 bar max. pressure
application(s)   HPLC: suitable
L × I.D.   7.5 cm × 2 mm
matrix   polymer particle platform
matrix active group   ether phase
particle size   10 μm
pore size   1000 Å
operating pH   2 - 12
mode of chromatography   Hydrophobic interaction (HIC)

Description

General description

Among the TSKgel HIC columns, TSKgel Ether-5PW has intermediate hydrophobity. It is an excellent choice for separating hydrophobic molecules such as membrane proteins or monoclonal antibodies such as IgG or IgM. Featuring a 1000 A pore size in a polymethacrylate base material, the Ether-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients.

Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Articles

HPLC Analysis of Mouse Monoclonal Antibodies (MAb) on TSKgel® Ether-5PW

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Mouse Monoclonal Antibodies (MAb) on TSKgel® Ether-5PW
Keywords: Chromatography, High performance liquid chromatography

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Related Products

guard cartridge

Product #

Description

Add to Cart

842156 TSKgel® Ether-5PW HPLC Column, phase ether, L × I.D. 1 cm × 2 mm, 10 μm particle size, pkg of 3 ea

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?