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820009 Supelco

TSKgel® Amide-80 HPLC Column

phase carbamoyl, L × I.D. 5 cm × 1 mm, 5 μm particle size

Synonym: TSKgel® Amide-80

  •  eCl@ss 32110501



material   stainless steel hardware
mfr. no.   Tosoh, 20009
parameter   0-100% organic solvent
  10-80 °C temperature
  30 bar max. pressure
application(s)   HPLC: suitable
L × I.D.   5 cm × 1 mm
matrix   silica particle platform
matrix active group   carbamoyl phase
particle size   5 μm
pore size   100 Å
surface area   450 m2/g
operating pH   2.0 - 7.5
suitability   suitable for L68 per USP
mode of chromatography   Hydrophilic interaction (HILIC)


Physical form

Shipped in 85% acetonitrile - 15% water.

General description

TSKgel Amide-80 columns are packed with 3, 5, or 10 micron spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 °C) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, the new, high efficiency, 3 micron columns offer shorter analysis time and enhanced sensitivity for LC-MS applications.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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