• USA Home
  • 820022 - TSKgel® BioAssist® Chelate HPLC Column

820022 Supelco

TSKgel® BioAssist® Chelate HPLC Column

phase iminodiacetic acid, L × I.D. 5 cm × 7.8 mm, 10 μm particle size, PEEK hardware

Synonym: TSKgel® Chelate-5PW HPLC Column and Guardgel Kit

  •  eCl@ss 32110501



Related Categories Analytical/Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, TSKgel Affinity HPLC Columns, TSKgel BioAssist PEEK HPLC Columns,
material   PEEK hardware
mfr. no.   Tosoh, 20022
extent of labeling   20 µmol/mL resin ligand density
parameter   ≤20% organic solvent
  ≤3 M salt concentration
  ≤45 °C temperature
  1.2 mL/min flow rate
  10 bar max. pressure
application(s)   HPLC: suitable
L × I.D.   5 cm × 7.8 mm
matrix   polymer particle platform
matrix active group   iminodiacetic acid phase
particle size   10 μm
pore size   1000 Å
operating pH   2 - 12
mode of chromatography   Affinity


General description

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.

Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

BioAssist is a registered trademark of Tosoh Corporation

TSKgel is a registered trademark of Tosoh Corporation

Price and Availability

All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis

Protocols & Articles

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?