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820022 Supelco

TSKgel® BioAssist® Chelate HPLC Column

phase iminodiacetic acid, L × I.D. 5 cm × 7.8 mm, 10 μm particle size, PEEK hardware

Synonym: TSKgel® Chelate-5PW HPLC Column and Guardgel Kit

  •  eCl@ss 32110501

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Properties

Related Categories Analytical/Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, TSKgel Affinity HPLC Columns, TSKgel BioAssist PEEK HPLC Columns,
material   PEEK hardware
mfr. no.   Tosoh, 20022
extent of labeling   20 µmol/mL resin ligand density
parameter   ≤20% organic solvent
  ≤3 M salt concentration
  ≤45 °C temperature
  1.2 mL/min flow rate
  10 bar max. pressure
application(s)   HPLC: suitable
L × I.D.   5 cm × 7.8 mm
matrix   polymer particle platform
matrix active group   iminodiacetic acid phase
particle size   10 μm
pore size   1000 Å
operating pH   2 - 12
mode of chromatography   Affinity

Description

General description

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.

Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.

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Legal Information

BioAssist is a registered trademark of Tosoh Corporation

TSKgel is a registered trademark of Tosoh Corporation

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Safety & Documentation

Safety Information

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Documents

Certificate of Analysis

Protocols & Articles

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