G002674 Supelco

HPLC Analysis of Steroids on Ascentis® RP-Amide (Mobile phase: 60:40)

application for HPLC

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Properties

Related Categories Analytical/Chromatography, Applications, HPLC Applications, Steroids
test parameters   column: Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)
  mobile phase: (A) water; (B) acetonitrile; (60:40, A:B)
  flow rate: 1 mL/min
  column temp.: 35 °C
  detector: UV, 254 nm
  injection: 10 μL
  sample: 50 μg/mL each in mobile phase
suitability   application for HPLC

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