|Related Categories||Analytical/Chromatography, Applications, HPLC Applications, Steroids|
|test parameters||column: Ascentis Phenyl, 15 cm x 4.6 mm I.D., 3 μm particles (581610-U)|
|mobile phase: (A) water: (B) acetonitrile; (75:25, A:B)|
|flow rate: 1.0 mL/min|
|column temp.: 35 °C|
|detector: UV, 240 nm|
|injection: 10 μL|
|sample: 50 μg/mL in 85:15, water:methanol|
|suitability||application for HPLC|
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