G003698 Supelco

HPLC Analysis of Steroids on Ascentis® Phenyl

application for HPLC

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Properties

Related Categories Analytical/Chromatography, Applications, HPLC Applications, Steroids
test parameters   column: Ascentis Phenyl, 15 cm x 4.6 mm I.D., 3 μm particles (581610-U)
  mobile phase: (A) water: (B) acetonitrile; (75:25, A:B)
  flow rate: 1.0 mL/min
  column temp.: 35 °C
  detector: UV, 240 nm
  injection: 10 μL
  sample: 50 μg/mL in 85:15, water:methanol
suitability   application for HPLC

Description

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Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

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Solone    
Hydrocortisone    
Prednisone    

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