G004540 Supelco

HPLC Analysis of Biotin Enantiomers on Astec® CHIROBIOTIC® T

application for HPLC

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Properties

Related Categories All Biochemical Applications, All Food & Beverage Applications, Analytical/Chromatography, Applications, Biochemicals,
test parameters   column: CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
  mobile phase: (A) 0.1% sodium phosphate, pH 4.1; (B) methanol; (60:40, A:B)
  flow rate: 1 mL/min
  column temp.: 23 °C
  detector: UV, 210 nm
  injection: 2 μL
  sample: 4 mg/mL in methanol
suitability   application for HPLC

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Astec is a registered trademark of Sigma-Aldrich Co. LLC

CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

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Biotin, ≥99% (TLC), lyophilized powder B4501  

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