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G002441 Supelco

HPLC Analysis of Peptide Mix, Complex, on Ascentis® C18

application for HPLC

Properties

Related Categories Analytical/Chromatography, Applications, HPLC Applications, Peptides
test parameters   column: Ascentis C18, 15 cm x 2.1 mm I.D., 5 μm particles (581304-U)
  mobile phase: [A] 25 mM formic acid in water; [B]: 50:50 (v/v) 25 mM formic acid in water:25 mM formic acid in acetonitrile
  gradient: 10 to 64% B in 90 min
  flow rate: 0.2 mL/min
  column temp.: 35 °C
  detector: ESI (+), full scan
  injection: 10 μL
  sample: tryptic digest of carboxymethylated protein mixture 2.4 g/L in 0.1% formic acid
suitability   application for HPLC
Featured Industry   Life Science and Biopharma

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