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G002613 Supelco

HPLC Analysis of Peptides on Ascentis® RP-Amide

application for HPLC

Properties

Related Categories Analytical/Chromatography, Applications, HPLC Applications, Peptides
test parameters   column: Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)
  mobile phase: [A] 0.1% trifluoroacetic acid in water; [B] 0.1% trifluoroacetic acid in acetonitrile
  gradient: 9 to 21% B in 5 min; to 27% B in 15 min
  flow rate: 1.0 mL/min
  column temp.: 30 °C
  detector: UV, 220 nm
  injection: 5 μL
  sample: as indicated in mobile phase A
suitability   application for HPLC
Featured Industry   Life Science and Biopharma

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Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

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