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G002877 Supelco

HPLC Analysis of Barbiturates on Ascentis® C18

application for HPLC

Properties

Related Categories Analytical/Chromatography, Anticonvulsant/antiepiletic/antiseizure, Anxiolytics, Sedatives, Applications, Forensics and Toxicology,
test parameters   column: Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
  mobile phase: [A] methanol; [B] water (50:50, A:B)
  flow rate: 1.0 mL/min
  column temp.: 35 °C
  detector: UV, 214 nm
  injection: 10 μL
  sample: as indicated in 65:35 water:methanol
suitability   application for HPLC
Featured Industry   Forensics and Toxicology
Pharmaceutical (small molecule)

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Description

Analysis Note

Barbiturates are commonly abused and among the most widely tested compounds in clinical, forensic, or therapeutic drug monitoring applications. Shown here is the baseline separation of a set of barbiturates on an Ascentis C18 column. Highest grade HPLC solvents were used to supply low background interference and low particulate contamination for robust, trouble-free operation. Cerilliant and Sigma-Aldrich reference standards provided reliable identification and quantification.

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Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

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Barbital SDS B0375  
Phenobarbital solution, 1 mg/mL in methanol, ampule of 1 mL, certified reference material SDS P-008  
Aprobarbital 50 μg/mL SDS    
Butabarbital solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material SDS B-024  
Mephobarbital, United States Pharmacopeia (USP) Reference Standard SDS 1386000  
Pentobarbital solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material SDS P-010  
Secobarbital solution, 1 mg/mL in methanol, ampule of 1 mL, certified reference material SDS S-002  
Secobarbital 50 μg/mL SDS    

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