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G002992 Supelco

HPLC Analysis of Ulcer Treatment Drugs on Ascentis® RP-Amide

application for HPLC

Properties

Related Categories Analytical/Chromatography, Antiulcer Compounds, Applications, HPLC Applications
test parameters   column: Ascentis RP-Amide, 15 cm x 2.1 mm I.D., 5 μm particles (565305-U)
  mobile phase: [A] 10 mM ammonium phosphate, pH 6.2 with phosphoric acid; [B] acetonitrile
  gradient: 5 to 15% B in 10 min; to 55% B in 8 min; held for 5 min
  flow rate: 0.2 mL/min
  column temp.: 35 °C
  detector: UV, 220 nm
  injection: 5 μL
  sample: as indicated in 95:5 water:methanol
suitability   application for HPLC
Featured Industry   Pharmaceutical (small molecule)

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Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

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