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G003006 Supelco

HPLC Analysis of Cold Remedy Ingredients on Ascentis® RP-Amide

application for HPLC

Properties

Related Categories Analytical/Chromatography, Antihistamines, Applications, HPLC Applications
test parameters   column: Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)
  mobile phase: [A] 5 mM ammonium formate, pH 2.75 with formic acid; [B] acetonitrile
  gradient: 10 to 30% B in 15 min; held for 2 min; to 10% B in 0.1 min
  flow rate: 1.0 mL/min
  column temp.: 30 °C
  detector: UV, 270 nm
  injection: 10 μL
  sample: as indicated in 5 mM ammonium formate (pH 2.75 with formic acid)
suitability   application for HPLC
Featured Industry   Pharmaceutical (small molecule)

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