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G003710 Supelco

HPLC Analysis of Nucleosides on Ascentis® Phenyl

application for HPLC

Properties

Related Categories Analytical/Chromatography, Applications, HPLC Applications, Nucleic Acids
test parameters   column: Ascentis Phenyl, 15 cm x 2.1 mm I.D., 5 μm particles (581613-U)
  mobile phase: 10 mM ammonium formate (pH 3.0 with formic acid)
  flow rate: 0.2 mL/min
  column temp.: 35 °C
  detector: UV, 270 nm; 750 psi back pressure regulator on outlet of flow cell
  injection: 1 μL
  sample: as indicated, in mobile phase
suitability   application for HPLC
Featured Industry   Life Science and Biopharma

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Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

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Cytidine, ≥99.0% (HPLC) SDS 30270  
2′-Deoxycytidine hydrate, ≥99% (HPLC) SDS 854980  
Guanosine, ≥98% SDS G6752  
Uridine, ≥99% SDS U3750  
Adenosine, ≥99% SDS A9251  
3′-Deoxyguanosine .1 mg/mL SDS D7285  
5′-Deoxyadenosine, methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase substrate .1 mg/mL SDS D1771  
Thymidine, ≥99.0% (HPLC) .1 mg/mL SDS 89270  

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