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G003716 Supelco

HPLC Analysis of Biogenic Amines on Ascentis® Si, HILIC Mode (85 % Acetonitrile)

application for HPLC

Properties

Related Categories Amines, Analytical/Chromatography, Applications, Clinical, HPLC Applications,
test parameters   column: Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
  mobile phase: [A] 0.1% ammonium acetate in water (pH unadjusted); [B] 0.1% ammonium acetate in acetonitrile; (15:85, A:B)
  flow rate: 1 mL/min
  column temp.: 35 °C
  detector: UV, 280 nm
  injection: 10 μL
  sample: 50 μg/mL in 0.1% ammonium acetate in 10:90:water:acetonitrile
suitability   application for HPLC
Featured Industry   Clinical

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Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

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