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G003722 Supelco

HPLC Analysis of Amino Acids, Polar Neutral and Acidic on Ascentis® Si

application for HPLC


Related Categories Amino Acid Analysis, Amino Acids, Analytical/Chromatography, Application Notes for amino acid analysis, Applications,
test parameters   column: Ascentis Si, 10 cm x 2.1 mm I.D., 5 μm particles (581508-U)
  mobile phase: [A] 100 mM ammonium formate, pH 3.0, with formic acid; [B] water; [C] acetonitrile
  gradient: 5% A constant; 10% B, 85% C to 16% B, 79% C in 20 min
  flow rate: 0.3 mL/min
  column temp.: 35 °C
  detector: ESI (+), full scan
  injection: 2 μL
  sample: as indicated, in 10:90, (50 mM ammonium formate/formic acid, (pH 3.0):acetonitrile
suitability   application for HPLC
Featured Industry   Life Science and Biopharma

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