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G003727 Supelco

HPLC Analysis of Vitamins, Fat Soluble, on Ascentis® Si

application for HPLC


Related Categories Analytical/Chromatography, Applications, Biochemicals and Reagents, Chromatographic Methods for Fat Soluble Vitamin Analysis, HPLC Applications,
test parameters   column: Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
  mobile phase: [A] hexane; [B] ethyl acetate
  gradient: 10 to 30% B in 10 min; held for 2 min; to 10% B in 1 min
  flow rate: 1.0 mL/min
  column temp.: 30 °C
  detector: UV, 290 nm
  injection: 10 μL
  sample: as indicated in 96:4 (hexane:isopropanol)
suitability   application for HPLC
Featured Industry   Vitamins, Nutraceuticals, and Natural Products

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