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G003728 Supelco

HPLC Analysis of Steroids on Ascentis® Si

application for HPLC

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Related Categories Analytical/Chromatography, Applications, Clinical, HPLC Applications, Steroids,
test parameters   column: Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
  mobile phase: [A] hexane; [B] ethanol; (88:12, A:B)
  flow rate: 1.0 mL/min
  column temp.: 35 °C
  detector: UV, 245 nm
  injection: 10 μL
  sample: 50 μg/mL in 85:15, hexane:2-propanol
suitability   application for HPLC
Featured Industry   Clinical
Vitamins, Nutraceuticals, and Natural Products

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Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

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