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HPLC Analysis of 25-Hydroxyvitamin D3 and epi-25-Hydroxyvitamin D3 on Ascentis® Express F5

application for HPLC

Properties

Related Categories Analytical/Chromatography, Applications, Biochemicals and Reagents, Chromatographic Methods for Fat Soluble Vitamin Analysis, Clinical,
test parameters   column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
  mobile phase: [A] 7 mM ammonium formate; [B] methanol; (25:75, A:B)
  flow rate: 0.4 mL/min
  column temp.: 40 °C
  detector: UV, 265 nm

ESI(+), m/z 100 - 1000
  injection: 1 μL
  sample: 300 mg/L in 25:75, water:methanol
suitability   application for HPLC
Featured Industry   Clinical
Vitamins, Nutraceuticals, and Natural Products

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25-Hydroxyvitamin D3 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material SDS H-086  
3-epi-25-Hydroxyvitamin D3, 98% (CP) SDS 705993  

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