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HPLC Analysis of Basic Peptides (Angiotensin Analogs) on BIOshell A160 Peptide C18

application for HPLC

Properties

Related Categories Analytical/Chromatography, Applications, HPLC Applications, Peptides
test parameters   column: BIOshell A160 Peptide C18, 10 cm x 3 mm I.D., 2.7 μm particles (66908-U)
  mobile phase: [A] 0.1% (v/v) formic acid, pH 4.0 (titrated with ammonium hydroxide); [B] 50:50, (0.175% formic acid in water, pH 4.0) : acetonitrile
  gradient: 20 to 50% B in 15 min
  flow rate: 0.6 mL/min
  pressure: 3002 psi (207 bar)
  column temp.: 35 °C
  detector: UV, 215 nm
  injection: 2.0 μL
  sample: 0.5 g/L ea. peptide
suitability   application for HPLC
Featured Industry   Life Science and Biopharma
Pharmaceutical (small molecule)

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