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G005391 Supelco

HPLC Analysis of Diet Soda Spiked with Neotame on Ascentis® Express RP-Amide

application for HPLC

Properties

Related Categories Analytical/Chromatography, Applications, Flavors, Fragrances, HPLC Applications
test parameters   column: Ascentis Express RP-Amide, 3 cm x 4.6 mm I.D., 2.7 μm particles (53921-U)
  mobile phase: [A] 100 mM ammonium acetate, pH 5.6 with acetic acid; [B] water; [C] acetonitrile
  gradient: 20% A constant; 75 to 20% B, 5 to 60% C in 1 min; held at 20% B, 60% C for 0.1 min
  flow rate: 3 mL/min
  pressure: 3336 psi (230 bar)
  column temp.: 40 °C
  detector: UV, 214 nm
  injection: 1 mL/min
  sample: diet soda spiked with neotame 350 μg/mL
suitability   application for HPLC
Featured Industry   Food and Beverages

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Description

Analysis Note

This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of common drink additives used in diet cola.

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