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G006143 Supelco

HPLC Analysis of Proteins and Peptides on Proteomix® WCX, Comparison of Different Particle Sizes

application for HPLC

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Related Categories Analytical/Chromatography, Applications, HPLC Applications, Peptides, Proteins More...
test parameters   column: Proteomix WCX NP3, 5 cm x 4.6 mm I.D., 3 μm particles (Z777185)
  column: Proteomix WCX-NP1.7, 5 cm x 4.6 mm I.D., 1.7 μm particles (Z777180)
  column: Proteomix WCX-NP10, 5 cm x 4.6 mm, 10 μm particles (Z777169)
  column: Proteomix WCX-NP5, 5 cm x 4.6 mm x 5 μm particles (Z777193)
  mobile phase: (A) 20 mM sodium phosphate, pH 6.5, (B) A + 1.0 M NaCl
  gradient: 0 to 100% B in 25 min, 15 min prewash
  flow rate: 0.5 mL/min
  column temp.: ambient
  detector: UV, 214 nm
  injection: 5 μL
  sample: Ribonuclease A, Cytochrome C and Lysozyme (1 mg/mL) min
suitability   application for HPLC
Featured Industry   Life Science and Biopharma
Pharmaceutical (small molecule)

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Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized SDS R6513  
Cytochrome c from chicken heart, ≥95% based on Mol. Wt. 12,222 basis SDS C0761  
Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein SDS L6876  

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