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G1006627 Supelco

UHPLC/MS/MS Analysis of 25 Small Molecule Pharmaceutical Compounds on Ascentis® Express RP-Amide

application for UHPLC-MS

Properties

Related Categories Analytical/Chromatography, Applications, HPLC Applications, Pharmaceuticals
test parameters   column: Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)
  mobile phase: [A] 0.1% acetic acid, 5 mM ammonium acetate in water; [B] 5 mM ammonium acetate in acetonitrile
  gradient: 5% B for 1 min; to 43% B in 6 min; held for 1 min; to 80% B in 4 min; held for 1 min; to 5% B in 1 min; held for 3 min
  flow rate: 0.4 mL/min
  detector: MS, ESI(+) MRM
  injection: 1 μL
suitability   application for UHPLC-MS
Featured Industry   Pharmaceutical (small molecule)

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Description

Antigen Background

This application highlights the advantage of an embedded polar group (EPG) HPLC phase for the separation of various small molecules. The use of an Ascentis® RP-Amide column allowed for the development of a single UPLC-MS/MS method for the analysis of 25 pharmaceuticals that varied significantly in both chemical and physical properties (log P, pKa, etc.). The presence of an embedded amide group allowed for retention of more polar analytes in comparison to a traditional C18 phase.

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Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

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Nizatidine, United States Pharmacopeia (USP) Reference Standard SDS 1467804  
Lincomycin hydrochloride, United States Pharmacopeia (USP) Reference Standard SDS 1367005  
Amiloride hydrochloride, United States Pharmacopeia (USP) Reference Standard SDS 1019701  
Hydrochlorothiazide, United States Pharmacopeia (USP) Reference Standard SDS 1314009  
Benzoylecgonine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material SDS B-004  
Imidacloprid, PESTANAL®, analytical standard SDS 37894  
Cinoxacin, United States Pharmacopeia (USP) Reference Standard SDS 1134109  
Mirtazapine, United States Pharmacopeia (USP) Reference Standard SDS 1444279  
Methapyrilene HCl, analytical standard SDS 442641  
Nevirapine anhydrous, United States Pharmacopeia (USP) Reference Standard SDS 1460703  
Imiquimod, United States Pharmacopeia (USP) Reference Standard SDS 1338313  
Hydroquinidine hydrochloride, 98% SDS 254819  
Buspirone hydrochloride, United States Pharmacopeia (USP) Reference Standard SDS 1078802  
Mesoridazine besylate, United States Pharmacopeia (USP) Reference Standard SDS 1393005  
Mianserin hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material SDS M-919  
Imipramine hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material SDS I-902  
Haloperidol solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material SDS H-030  
Amitriptyline hydrochloride solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material SDS A-923  
Clarithromycin, United States Pharmacopeia (USP) Reference Standard SDS 1134379  
Atrazine, certified reference material, TraceCERT® SDS 90935  
Losartan potassium, United States Pharmacopeia (USP) Reference Standard SDS 1370462  
Nefazodone hydrochloride, United States Pharmacopeia (USP) Reference Standard SDS 1457902  
4-Aminobiphenyl, analytical standard SDS 31598  
Nifedipine, United States Pharmacopeia (USP) Reference Standard SDS 1463508  
Loratadine, United States Pharmacopeia (USP) Reference Standard SDS 1370270  

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