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G1006629 Supelco

GC Analysis of Pesticides in Spinach on SLB®-5ms after QuEChERS Extraction then SPE Clean-up using Supel Sphere Carbon/NH2

application for GC, application for SPE


Related Categories Analytical/Chromatography, Applications, Capillary GC Columns and Guard Columns/Retention Gaps, Capillary GC Columns, by industry / application, Capillary GC Columns, by name,
test parameters   sample preparation: Solid Phase Extraction (SPE)
  sample/matrix: spinach extract
  SPE tube/cartridge: Supel Sphere Carbon/NH2 SPE Cartridge, 6 mL (54283-U)
  extraction process: to 10 g homogenized spinach add 10 mL acetonitrile and shake 1 min, add contents of Supel QuE acetate tube (55234-U), shake 1 min and centrifuge, transfer extract to dSPE tube, (add 1 g anhydrous MgSO4, shake 1 min and centrifuge, transfer 5 mL extract to glass tube, evaporate to 1 mL, add 250 μL toluene for a final volume of 1.25 mL QuEChERS extract)
  condition: 10 mL acetonitrile:toluene (75:25), discard
  sample addition: 1.25 mL QuEChERS extract
  elution: 20 mL acetonitrile:toluene (75:25) (gravity elution)
  eluate post-treatment: adjust volume of sample extract to 21.25 mL
  column: SLB-5ms, 20 m x 0.18 mm I.D., 0.36 μm (28576-U)
  oven: 50 °C (0.5 min), 25 °C/min to 125 °C (0 min), 10 °C/min to 300 °C (5 min)
  inj. temp.: 60 °C (0.28 min), 600 °C/min to 325 °C (5 min)
  detector: MSD
  MSD interface: 325 °C
  carrier gas: helium
  injection: 10 μL, PTV solvent vent, purge flow to split vent 60 mL/min at 2.78 min, vent flow 100 mL/min
  liner: 4 mm I.D., split/splitless type, wool packed single taper FocusLiner design
  sample: spinach extract
suitability   application for GC
  application for SPE
Featured Industry   Agriculture
Food and Beverages

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Analysis Note

Pigments, sterols, sugars, organic acids and fatty acids when extracted from leafy greens such as spinach, pose problems for long term GC performance. An effective clean-up technique is required that will isolate/retain matrix interferences without effecting the pesticide analytes of interest. Solid Phase Extraction is a common and effecient approach to effectivly clean-up extracts for low concentration residue analysis while reducing interferences. A 6 mL Supel Sphere Carbon/ NH2 (54283-U) SPE tube is used for fast, consistant gravity filtration clean-up of spinach extract for GC/MS analysis and produces good recoveries with low background.

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SLB is a registered trademark of Sigma-Aldrich Co. LLC

Supel is a trademark of Sigma-Aldrich Co. LLC



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alpha-BHC SDS    
Thiometon SDS    
Hexachlorobenzene SDS    
Dimethoate SDS    
Simazine SDS    
Quintozene SDS    
gamma-BHC SDS    
Toclofos-methyl SDS    
Heptachlor SDS    
Fenitrothion SDS    
Malathion SDS    
Metolachlor SDS    
Chlorophyrifos SDS    
Endosulfan I SDS    
Oxadiazon SDS    
Ethion SDS    
Triazophos SDS    
4,4′-DDT SDS    
Phosmet SDS    
Methoxychlor SDS    
Coumaphos SDS    
Cyfluthrin Isomers SDS    
Cypermethrin Isomers SDS    
Deltamethrin SDS    

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