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G1006630 Supelco

LC/MS/MS Analysis of Marine Toxins in Shellfish According to EU Regulations on Ascentis® Express C18

application for LC-MS

Properties

Related Categories Analytical/Chromatography, Applications, HPLC Applications, Natural products
test parameters   column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2 μm particles (50813-U)
  mobile phase: [A] 2mM ammonium acetate with 0.1% formic acid in acetonitrile:water (5:95), [B] 2 mM ammonium acetate with 0.1% formic acid in acetonitrile:water (95:5)
  gradient: 20% to 80% B in 4 min, to 95% B in 1 min, held at 95% B for 1 min, to 20% B in 0.5 min, held at 20% B for 1.5 min
  flow rate: 0.6 mL/min
  pressure: 5801 psi (400 bar)
  column temp.: 40 °C
  detector: MS/MS ESI(+) and ESI(-), MRM (see Figure for transitions)
  injection: 10 μL
suitability   application for LC-MS
Featured Industry   Forensics and Toxicology

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Description

Analysis Note

Biotoxins in shellfish have recently come under increased scrutiny. The European Union (EU) has issued regulation changes requiring all shellfish commodities to be tested for levels of these toxins. Shown here is a rapid and reproducible method for the extraction and analysis of lipophilic marine toxins from shellfish. Separation was achieved in less than seven minutes on an Ascentis® Express C18 column. Recoveries ranged from 80-120% using matrix-matched calibration standards. Certified reference materials provided reliable quantification.

Other Notes

mussel tissue homogenate matrix CRM (National Research Council Canada, part no. CRM-ZERO-Mus) extracted with methanol

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Azaspiracid-1 solution, 1.47 μM in methanol, certified reference material SDS    

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