F2502
Aldrich

Fluorescein isothiocyanate isomer I

90%

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1 of 1

Synonym

FITC

Fluorescein 5-isothiocyanate

Molecular Formula

C₂₁H₁₁NO₅S

Molecular Weight

389.38

CAS Number

3326-32-7

Beilstein Registry Number

1407295

MDL number

MFCD00005063

PubChem Substance ID

24894823

EG/EC Number

222-042-0

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Descriptions

Application

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Packaging

1 g in glass btl

50, 250 mg in glass btl

Application

Fluorescent labeling reagent for proteins.^1,^2,^3,^4,⁵ Used in the fluorescent antibody technique for rapid identification of pathogens.^6,⁷

Properties

assay

90%

fluorescence

λ_ex 492 nm; λ_em 518 nm (green)

>360 °C(lit.)

UV absorption

λ_max 499 nm

References

Cited Reference

1. Nature 193, 167, (1962)

2. Methods Enzymol. 26, 28, (1972) abstract

3. Anal. Biochem. 57, 227, (1974) abstract

4. Proc. Natl. Acad. Sci. USA 72, 459, (1975) abstract

5. Fluorescent Protein Tracing Baltimore, MD , (1962)

6. Am. J. Pathol. 34, 1081, (1958) abstract

7. Stain Technol. 44, 179, (1969) abstract

Reference

Muramoto, K., Microsequencing of proteins and peptides (HPLC). Anal. Biochem. 141, 446, (1984)

Wilderspin, A.F., Green, N.M., Modification of thiol groups. Anal. Biochem. 132, 449, (1983)

Der-Balian, G.P., FITC labelling of Fab'. Anal. Biochem. 173, 59, (1988)

Miller, L., Protein modification of actin at lysine-61. Eur. J. Biochem. 174, 23, (1988)

reference

Aldrich MSDS 1, 948:C / Corp MSDS 1 (1), 1688:D / FT-IR 2 (3), 4153:C / FT-IR 1 (2), 469:C / FT-NMR 1 (2), 1560:B / IR-Spectra (3), 1142:D / IR-Spectra (2), 995:C / NMR-Reference 2 (2), 440:C / RegBook 1 (2), 2157:I / Sigma FT-IR 1 (2), 148:B / Stains and Dyes Ref, 377 / Structure Index 1, 445:C:5

Safety

Hazard Codes

Risk Statements

42/43

Safety Statements

22-36/37-45

WGK Germany

Related Categories

... Stains & Dyes, A to Z > E-F

... Fluorescent Labels > Fluorescein and Derivatives

FT-IR Raman

FT-NMR

MSDS

Specification Sheet

Certificate of Analysis

More Information