01662
Fluka

Acridine Orange hydrochloride hydrate

purum, for microscopy (Fl., Hist.), ≥98.0% (HPLC)

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Synonym

3,6-Bis(dimethylamino)acridine hydrochloride

Molecular Formula

C₁₇H₁₉N₃ · HCl · xH₂O

Molecular Weight

301.81 (anhydrous basis)

CAS Number

65-61-2

Colour Index Number

46005

Beilstein Registry Number

3734978

MDL number

MFCD00150070

PubChem Substance ID

24844901

EG/EC Number

200-614-0

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Descriptions

Application

Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.

Properties

grade

for microscopy (Fl., Hist.)

purum

assay

≥98.0% (HPLC)

284-287 °C(lit.)

References

Reference

Erenpreisa, J., et al., Apoptotic cell nuclei favour aggregation and fluorescence quenching of DNA dyes. Histochem. Cell Biol. 108, 67-75, (1997)

Pulliam, L., et al., Quantification of neurotoxicity and identification of cellular subsets in a three-dimensional brain model. Cytometry 32, 66-69, (1998)

Carmichael, G.G., and McMaster, G.K., The analysis of nucleic acids in gels using glyoxal and acridine orange. Meth. Enzymol. 65, 380-391, (1980)

Mpoke, S.S., and Wolfe, J., Differential staining of apoptotic nuclei in living cells: application to macronuclear elimination in Tetrahymena. J. Histochem. Cytochem. 45, 675-683, (1997)

Darzynkiewicz, Z., et al., Features of apoptotic cells measured by flow cytometry. Cytometry 13, 795-808, (1992)

Beilstein

Beil. 22,V,11,326

reference

FT-IR 2 (3), 3939:B / RegBook 1 (2), 2637:G / Sax 6, 474 / Stains and Dyes Ref, 63 / Structure Index 1, 415:E:7

Safety

Safety Statements

22-24/25

WGK Germany

RTECS

AR7601000