Acylase I from porcine kidney 

Review¹; Characterization²

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1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-acetyl-L-methionine per minute at pH 7.0 and 25°C

The enzyme hydrolyzes a variety of N-acyl-L-amino acids and dehydropeptides containing dehydroalanyl residues. N-acyl-D-amino acids and dehydropeptides other than dehydroalanine are not hydrolyzed. The following compounds are hydrolyzed by acylase I: chloroacetyl-L-amino acids, L-leucinamide, chloroacetyldehydroalanine, glycyl-L-amino acids, and glycyldehydroalanine. Aminoacylase is widely used as a reagent to resolve amino acid racemates. The K_m for acyl amino acids have been reported in the literature: Ac-L-Met (0.99 mM), Ac-L-Glu (10.2 mM), and Ac-L-Phe (5.5 mM).

Aminoacylase from pig kidney is activated by Zn²⁺ and Co²⁺ and inhibited by SH blocking reagents, disulfide reducing reagents, N-tosyl-L-alanine,
DL-norleucine, and N-tosyl-L-lysine chloromethylketone.

BioChemika

powder

M_r ~45300

yellow-brown

pig ... ACY1(396930)

−20°C

1. F. Schneider Wiss. Beitr.- Martin Luther Univ. Halle-Wittenberg 3, Ber., 203, (1988)

2. D. Heese et al. Hoppe Seyler's Z. Physiol. Chem. 369, 559, (1988)

1

BF4890000