High throughput crystallization screening kit for Nucleic acids 

The high throughput crystallization kit for nucleic acids is an empirical screening method for the direct determination of suitable crystallization conditions for nucleic acids.
The kit is developed according to the described methods of Nowakowski, et al.¹ and Baeyens, Jancarik and Holbrook².The solutions and crystallization conditions are empirically derived based on known or published crystallization conditions of nucleic acids, including DNA enzymes or other complex nucleic acids (Ref. 3-6), as well as RNA or other short oligonucleotices.
The high throughput crystallization kit for nucleic acids includes 96 solutions that are pre-formulated for ease of use. They cover a range of different buffers (pH 5.5-8.0), different types of precipitants (esp. PEG, MPD, ethanol, 2-propanol), and inorganic salts including magnesium salts. All solutions are sterile filtered using 0.22 micron filters. The kit includes 1 mL of each pre-formulated solution filled in a deepwell plate. This standard format makes automated liquid handling very easy. But also for manual filling of crystallization plates the use of multi-channel pipettes saves much time.

BioChemika

2-8°C

1. Nowakowski, J., et al., Crystallization of the 10-23 DNA enzyme using a combinatorial screen of paired oligonucleotides Acta Crystallogr. D (Biol. Crystallogr.) 55, 1885-1892, (1999) abstract

2. Baeyens, K.J., et al., Use of Low-Molecular-Weight Polyethylene Glycol in the Crystallization of RNA Oligomers Acta Crystallogr. D (Biol. Crystallogr.) 50, 764-767, (1994) abstract

UN 3316 6.1/PG 3