N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride 

E1769, N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

Peptide activation and coupling to solid supports under conditions favouring selective activation of the terminal carboxyl group¹

Water soluble condensing reagent. It is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. It has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, it is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

for protein sequence analysis

BioChemika

110-115 °C(lit.)

−20°C

1. A. Previero et al. FEBS Lett. 33, 135, (1973)

Lundblad, R.L., et al., Modification of carboxyl groups in proteins, review. Chemical Reagents for Protein Modification 2, 105, (1984)

Thomas, J.O., et al., Altered arrangement of the DNA in injection-defective lambda bacteriophage. J. Mol. Biol. 123, 149, (1978)

Sheehan, J.C., and Ledis, Total synthesis of a monocyclic peptide lactone antibiotic, etamycin. J. Am. Chem. Soc. 95, 875-879, (1973)

Drabick, J.J., et al., Covalent polymyxin B conjugate with human immunoglobulin G as an antiendotoxin reagent. Antimicrob. Agents Chemother. 42, 583-588, (1998)

Dhaon, M.K., Synthesis of esters. J. Org. Chem. 47, 1962, (1982)

3

FF2200000