Aurintricarboxylic acid ammonium salt 

for the determination of Al, Ga, Se, Th

Aurintricarboxylic acid readily polymerizes in aqueous solution, forming a stable free radical that inhibits protein-nucleic acid interactions¹. It is a potent inhibitor of ribonuclease¹ and topoisomerase II² by preventing the binding of the nucleic acid to the enzyme. It stimulates tyrosine phosphorylation processes including the Jak2/STAT5 pathway in NB2 lymphoma cells³, ErbB4 in neuroblastoma cells⁴, and MAP kinases, Shc proteins, phosphatidylinositide 3-kinase and phospholipase C in PC12 cells⁵. Aurintricarboxylic acid inhibits apoptosis in many cell types. Its neuroprotective effect, perhaps due to its ability to prevent down-regulation of Ca²⁺ impermeable GluR2 receptors⁶ or to its ability to inhibit calpain, a Ca²⁺-activated protease that is activated during apoptosis⁷.

May contain a substantial amount of polymeric material.

ACS reagent

p.a.

~80% (T)

for the determination of Al, F^-

≤0.2% (as SO₄)

220-225 °C (dec.)(lit.)

Gonzalez, R.G., et al., Mechanism of action of polymeric aurintricarboxylic acid, a potent inhibitor of protein--nucleic acid interactions. Biochemistry 19, 4299-4303, (1980)

Okada, N., and Koizumi, S., A neuroprotective compound, aurintricarboxylic acid, stimulates the tyrosine phosphorylation cascade in PC12 cells. J. Biol. Chem. 270, 16464, (1995)

Posner, A., et al., Aurintricarboxylic acid is an inhibitor of mu- and m-calpain. Biochem. Mol. Biol. Int. 36, 291-299, (1995)

Aronica, E.M., et al., Aurintricarboxylic acid prevents GLUR2 mRNA down-regulation and delayed neurodegeneration in hippocampal CA neurons of gerbil after global ischemia. Proc. Natl. Acad. Sci. USA 95, 7115-7120, (1998)

Okada, N., and Koizumi, S., Tyrosine phosphorylation of ErbB4 is stimulated by aurintricarboxylic acid in human neuroblastoma SH-SY5Y cells. Biochem. Biophys. Res. Commun. 230, 266-269, (1997)

Benchokroun, Y., et al., Aurintricarboxylic acid, a putative inhibitor of apoptosis, is a potent inhibitor of DNA topoisomerase II in vitro and in Chinese hamster fibrosarcoma cells. Biochem. Pharmacol. 49, 305-313, (1995)

Rui, H., et al., Activation of the Jak2-Stat5 signaling pathway in Nb2 lymphoma cells by an anti-apoptotic agent, aurintricarboxylic acid. J. Biol. Chem. 273, 28-32, (1998)

Structure Index 1, 299:B:7

Xi

36/38

26-36

2

GU4800000