Fluorescent IEF-Marker pI 10.3 

suitable as IEF-Marker for HPCE and for IEF-gels

Dissolve the IEF-marker at 1mg/ml in H₂O or 10 mM HCl, sonicate for up to 10 min if necessary. Store at 4 °C (the solution is stable for approximately 6 months). For use in HPCE this stock solution should be diluted 1:100 in a suitable ampholyte solution (e.g. 2% ampholyte 3-10), UV-detection at 280 nm (20 °C). For use in IEF-gels the 1 mg/ml stock solution can be loaded directly onto the gel (max. 1 μl per lane).

BioChemika

λ_ex 388 nm; λ_em 495 nm in 0.1 M carbonate pH 10.3

2-8°C

3

77672, Fluorescent IEF-Marker pI 10.3 solution