20966
Fluka

Cesium chloride

BioUltra, for molecular biology, ≥99.5% (AT)

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Molecular Formula

CsCl

Molecular Weight

168.36

CAS Number

7647-17-8

MDL number

MFCD00010955

PubChem Substance ID

24852585

EG/EC Number

231-600-2

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Descriptions

Application

Used for the preparation of electrically conducting glasses.^1,² Used to make solutions for the separation of RNA from DNA by density gradient centrifugation.³

Other Notes

Cesium chloride gradient centrifugation for phage purification;⁴ Isolation of satellite DNA;⁵ RNA purification;⁶ Buoyant density of DNA - Hoechst 33258 complexes in CsCl gradients.⁷

Properties

grade

for molecular biology

product line

BioUltra

assay

≥99.5% (AT)

total impurities

DNases, none detected

RNases, none detected

insoluble matter, passes filter test

phosphatases, none detected

proteases, none detected

≤0.001% total nitrogen (N)

loss

≤0.1% loss on drying, 110 °C

6.0-7.5 (25 °C, 3 M in H₂O)

645 °C(lit.)

solubility

H₂O: 3 M at 20 °C, clear, colorless

anion traces

sulfate (SO₄^2-): ≤20 mg/kg

cation traces

Al: ≤5 mg/kg

As: ≤0.1 mg/kg

Ba: ≤5 mg/kg

Bi: ≤5 mg/kg

Ca: ≤50 mg/kg

Cd: ≤5 mg/kg

Co: ≤5 mg/kg

Cr: ≤5 mg/kg

Cu: ≤5 mg/kg

Fe: ≤5 mg/kg

K: ≤50 mg/kg

Li: ≤5 mg/kg

Mg: ≤5 mg/kg

Mn: ≤5 mg/kg

Mo: ≤5 mg/kg

Na: ≤50 mg/kg

Ni: ≤5 mg/kg

Pb: ≤5 mg/kg

Sr: ≤5 mg/kg

Zn: ≤5 mg/kg

3 M in H₂O

UV absorption

λ: 260 nm A_max: 0.02

λ: 280 nm A_max: 0.02

References

Cited Reference

1. Tver’yanovich, Y.S. et al. Glass Phys. Chem. 24, 446, (1998)

2. J. Am. Ceram. Soc. 90, 1822, (2007)

3. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, , (1989), 7.19-7.22

4. Miller, H. Meth. Enzymol. 152, 159, (1987)

5. Cooney, C. A., Matthews, H. R. Methods Mol. Biol. 2, 21, (1984)

6. Turpen, T. H. and Griffith, O. M. Biotechnology 4, 11, (1986)

7. P. Karlovsky, P., and de Cock, A. W. Anal. Biochem. 194, 192, (1991) abstract

Merck

Merck 13,2021

reference

Aldrich MSDS 1, 362:C / Corp MSDS 1 (1), 716:A / RegBook 1 (3), 3319:J / Sax 6, 661

Safety

WGK Germany

RTECS

FK9625000

Related Categories

... Density Gradient > Alphabetical

... Molecular Biology Reagents > BioUltra

... DNA & RNA Purification > Reagents

... Density Gradient > Salts of Alkali Metals

MSDS

Certificate of Analysis

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