38440
Fluka

4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid disodium salt hydrate

BioChemika, for fluorescence, ≥75% (S)

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Synonym

DIDS

Disodium 4,4'-diisothiocyanatostilbene-2,2'-disulfonate

Molecular Formula

C₁₆H₈N₂Na₂O₆S₄ · xH₂O

Molecular Weight

498.48 (anhydrous basis)

CAS Number

207233-90-7

Beilstein Registry Number

8179433

MDL number

MFCD00009638

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Descriptions

Application

A negatively charged homobifunctional cross-linking reagent. The isothiocyanate groups react with primary amines at pH 9.0-10.0.

Biochem/physiol Actions

A specific inhibitor of cellular anion permeability used in cell transport studies.

Other Notes

Fluorescent reagent for the modification of proteins¹

Properties

grade

for fluorescence

product line

BioChemika

assay

≥75% (S)

fluorescence

λ_ex 341 nm; λ_em 415 nm in 0.1 M phosphate pH 7.0

storage temp.

2-8°C

References

Cited Reference

1. I.Z. Cabantchik, A. Rothstein J. Membr. Biol. 10, 311, (1972) abstract

Reference

Pusch, M., et al., Channel or transporter? The CLC saga continues. Exp. Physiol. 91, 149-152, (2006)

Okada, Y., et al., Volume-sensitive chloride channels involved in apoptotic volume decrease and cell death. J. Membr. Biol. 209, 21-29, (2006)

Alper, S.L., Molecular physiology of SLC4 anion exchangers. Exp. Physiol. 91, 153-161, (2006)

Kavanaugh, M.P., et al., Affinity labeling of hemoglobin with 4,4'-diisothiocyanostilbene-2,2'-disulfonate: covalent cross-linking in the 2,3-diphosphoglycerate binding site. Biochemistry 27, 1804-1808, (1988)

Hua, S., and Inesi, G., Lys515-Lys492 cross-linking by DIDS interferes with substrate utilization by the sarcoplasmic reticulum ATPase. Biophys. J. 73, 2149-2155, (1997)

Safety

Hazard Codes

Risk Statements

36/37/38-42

Safety Statements

22-26

WGK Germany

3-10-21