Analysis Note
free of unconjugated dye
General description
Anti-rabbit IgG (whole molecule) (Sigma, R4880) is developed in goat using purified rabbit IgG as the immunogen. Affinity isolated antigen specific antibody is purified from goat anti-rabbit IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to rabbit IgG. Goat anti-rabbit is conjugated to Atto 633 NHS (Abs.max. 629 nm; Em.max. 657 nm), (Fluka 01464), then further purified via gel permeation chromatography and dialysis to remove unbound Atto-Dye.