43794
Fluka

1,4-Dithioerythritol

BioUltra, ≥99.0% (RT)

zoom

1 of 1

Synonym

erythro-1,4-Dimercapto-2,3-butanediol

erythro-2,3-Dihydroxy-1,4-butanedithiol

Cleland’s reagent

DTE

Molecular Formula

HSCH₂CH(OH)CH(OH)CH₂SH

Molecular Weight

154.25

CAS Number

6892-68-8

Beilstein Registry Number

1719756

MDL number

MFCD00063750

PubChem Substance ID

24867409

EG/EC Number

229-998-8

Expand/Collapse All

Descriptions

Application

Reagent for maintaining −SH groups in the reduced state; quantitatively reduces disulfides.

Other Notes

Cleavage of disulfide bonds in proteins;^1,² Prevents oxidation of sulfhydryl-containing proteins during SDS-polyacrylamide gel electrophoresis.³

Properties

product line

BioUltra

assay

≥99.0% (RT)

total impurities

insoluble matter, passes filter test

≤0.5% 4,5-dihydroxy-1,2-dithiane

4.0-6.0 (25 °C, 0.1 M in H₂O)

82-84 °C(lit.)

82-85 °C

solubility

H₂O: 0.1 M at 20 °C, clear, colorless

anion traces

sulfate (SO₄^2-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg

As: ≤0.5 mg/kg

Ba: ≤5 mg/kg

Bi: ≤5 mg/kg

Ca: ≤50 mg/kg

Cd: ≤5 mg/kg

Co: ≤5 mg/kg

Cr: ≤5 mg/kg

Cu: ≤5 mg/kg

Fe: ≤5 mg/kg

K: ≤50 mg/kg

Li: ≤5 mg/kg

Mg: ≤5 mg/kg

Mn: ≤5 mg/kg

Mo: ≤5 mg/kg

Na: ≤50 mg/kg

Ni: ≤5 mg/kg

Pb: ≤5 mg/kg

Sr: ≤5 mg/kg

Zn: ≤5 mg/kg

0.1 M in H₂O

UV absorption

λ: 260 nm A_max: 0.400

λ: 280 nm A_max: 0.050

storage temp.

2-8°C

References

Cited Reference

1. W.L. Zahler, W.W. Cleland J. Biol. Chem. 243, 716, (1968) abstract

2. U.T. Rüegg, J. Rudinger Meth. Enzymol. 47, 111, (1977) abstract

3. L.C. Lane Anal. Biochem. 86, 655, (1978)

Reference

U.T. Rüegg, J. Rudinger Methods Enzymol. 47, 111, (1977) abstract

Beilstein

Beil. 1,III,2359

reference

Aldrich MSDS 1, 839:D / Corp MSDS 1 (1), 1482:B / FT-IR 2 (1), 418:A / FT-IR 1 (1), 263:D / FT-NMR 1 (1), 424:B / IR-Spectra (3), 155:F / IR-Spectra (2), 139:B / NMR-Reference 2 (1), 230:C / RegBook 1 (1), 283:D / Sigma FT-IR 1 (2), 533:B / Structure Index 1, 38:A:9 / Vapor Phase 3, 346:C

Safety

Hazard Codes

Risk Statements

36/37/38

Safety Statements

26-36

WGK Germany

RTECS

KF2410000

3-10-13-23