43819
Fluka

DL-Dithiothreitol

BioChemika, ≥99.0% (RT)

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Synonym

threo-1,4-Dimercapto-2,3-butanediol

Cleland’s reagent

DTT

Molecular Formula

HSCH₂CH(OH)CH(OH)CH₂SH

Molecular Weight

154.25

CAS Number

3483-12-3

Beilstein Registry Number

1719757

MDL number

MFCD00004877

EG/EC Number

222-468-7

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Descriptions

Application

An excellent reagent for maintaining SH groups in reduced state;?? quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Properties

product line

BioChemika

assay

≥99.0% (RT)

total impurities

≤0.3% 4,5-dihydroxy-1,2-dithiane

41-44 °C(lit.)

42-43 °C

solubility

H₂O: 50 mg/mL, clear, colorless

anion traces

sulfate (SO₄^2-): ≤50 mg/kg

cation traces

Ca: ≤10 mg/kg

Cd: ≤5 mg/kg

Co: ≤5 mg/kg

Cr: ≤5 mg/kg

Cu: ≤5 mg/kg

Fe: ≤5 mg/kg

K: ≤50 mg/kg

Mg: ≤5 mg/kg

Mn: ≤5 mg/kg

Na: ≤200 mg/kg

Ni: ≤5 mg/kg

Pb: ≤5 mg/kg

Zn: ≤5 mg/kg

storage temp.

2-8°C

References

Reference

Cleland, W.W. Biochemistry 3, 480, (1964) abstract

Sears, D.W., In the investigation of incremental and kinetic reduction in proteins. Biochemistry 16, 2031, (1977)

Bacarese-Hamilton, A., Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues. Ann. N.Y. Acad. Sci. 448, 571, (1985)

Jabusch, J.R., In protein sequence analysis. Anal. Biochem. 91, 532, (1978)

MacDonald, R.J., Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds. Meth. Enzymol. 152, 219, (1987)

Ruegg, U.T., Cleavage of disulfide bonds in proteins. Meth. Enzymol. 47, 111, (1977)

Merck

Merck 13,3412

Beilstein

Beil. 1,III,2360

reference

Aldrich MSDS 1, 840:C / Corp MSDS 1 (1), 1483:A / FT-IR 2 (1), 417:D / FT-IR 1 (1), 263:C / FT-NMR 1 (1), 424:A / IR-Spectra (3), 155:E / IR-Spectra (2), 139:A / NMR-Reference 2 (1), 230:B / RegBook 1 (1), 283:C / Sigma FT-IR 1 (2), 534:A / Structure Index 1, 38:E:8

Safety

Hazard Codes

Risk Statements

22-36/37/38

Safety Statements

26-36

WGK Germany

RTECS

EK1610000

Flash Point(F)

235.4

Flash Point(C)