Fast Green FCF 

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Reagent for staining proteins after PAGE¹; Especially suitable in isoelectric focusing (does not bind to ampholytes)²

Standard Fluka

290 °C (dec.)(lit.)

1. C.M. Wilsau Anal. Biochem. 96, 263, (1979) abstract

2. R.E. Allen et al. Anal. Biochem. 104, 494, (1980) abstract

Wilson, C.M., Staining of proteins on gels: comparisons of dyes and procedures. Meth. Enzymol. 91, 236-247, (1983)

Allen, R.E., et al., Staining protein in isoelectric focusing gels with fast green. Anal. Biochem. 104, 494-498, (1980)

Merck 13,3970

Beil. 16,II,513

Aldrich MSDS 1, 945:D / Corp MSDS 1 (1), 1678:A / FT-IR 2 (3), 4134:B / FT-IR 1 (2), 1033:C / IR-Spectra (3), 1485:D / RegBook 1 (2), 2779:I / Sax 6, 1411 / Sigma FT-IR 1 (2), 141:B / Stains and Dyes Ref, 345 / Structure Index 1, 442:B:2

2

BQ4425000

F7258, Fast Green FCF