Ethidium bromide solution 

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

for fluorescence

BioChemika

~1% in H₂O

λ_ex 530 nm; λ_em 600 nm in 50 mM phosphate buffer pH 7.0 (upon binding to DNA)

2-8°C

, Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY , (1989) 6-44, 6.15

Severini, A. and Morgan, A.R., An assay for proteinases and their inhibitors based on DNA/ethidium bromide fluorescence. Anal. Biochem. 193, 83-89, (1991)

Moore, S.P. and Sutherland, B.M., A densitometric nondestructive microassay for DNA quantitation. Anal. Biochem. 144, 15-19, (1985)

El-Hamalawi, A.-R. A. et al., The fluorometric determination of nucleic acids in pea seeds by use of ethidium bromide complexes. Anal. Biochem. 67, 384-391, (1975)

Scaria, P.V. and Shafer, R.H., Binding of ethidium bromide to a DNA triple helix. Evidence for intercalation. J. Biol. Chem. 266, 5417-5423, (1991)

Karsten, U. and Wollenberger, A., Improvements in the ethidium bromide method for direct fluorometric estimation of DNA and RNA in cell and tissue homogenates. Anal. Biochem. 77, 464-470, (1977)

Merck 13,4751

Beil. 22,V,11,352

UN 2810 6.1/PG 3

2

SF7950000