46951
Fluka

Fluorescein isothiocyanate isomer I

BioChemika, ≥97.5% (HPLC)

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Synonym

FITC

Fluorescein 5-isothiocyanate

Molecular Formula

C₂₁H₁₁NO₅S

Molecular Weight

389.38

CAS Number

3326-32-7

Beilstein Registry Number

1407295

MDL number

MFCD00005063

EG/EC Number

222-042-0

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Descriptions

Application

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Properties

product line

BioChemika

assay

≥97.5% (HPLC)

total impurities

≤1.5% Isomer II

fluorescence

λ_ex 492 nm; λ_em 518 nm (green)

λ_ex 492 nm; λ_em 518 nm in 0.1 M phosphate pH 8.0

>360 °C(lit.)

storage temp.

2-8°C

References

Reference

Miller, L., Protein modification of actin at lysine-61. Eur. J. Biochem. 174, 23, (1988)

Der-Balian, G.P., FITC labelling of Fab'. Anal. Biochem. 173, 59, (1988)

Wilderspin, A.F., Green, N.M., Modification of thiol groups. Anal. Biochem. 132, 449, (1983)

Muramoto, K., Microsequencing of proteins and peptides (HPLC). Anal. Biochem. 141, 446, (1984)

reference

Aldrich MSDS 1, 948:C / Corp MSDS 1 (1), 1688:D / FT-IR 2 (3), 4153:C / FT-IR 1 (2), 469:C / FT-NMR 1 (2), 1560:B / IR-Spectra (3), 1142:D / IR-Spectra (2), 995:C / NMR-Reference 2 (2), 440:C / RegBook 1 (2), 2157:I / Sigma FT-IR 1 (2), 148:B / Stains and Dyes Ref, 377 / Structure Index 1, 445:C:5

Safety

Hazard Codes

Risk Statements

42/43

Safety Statements

22-36/37-45

WGK Germany

Related Categories

... Fluorescent Labels > Fluorescein and Derivatives

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MSDS

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