47671
Fluka

Formamide

BioUltra, for molecular biology, ≥99.5% (T)

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Synonym

Amide C₁

Formic amide

Molecular Formula

HCONH₂

Molecular Weight

45.04

CAS Number

75-12-7

Beilstein Registry Number

505995

MDL number

MFCD00007941

PubChem Substance ID

24871294

EG/EC Number

200-842-0

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Descriptions

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Other Notes

For the preparation of formamide denaturing polyacrylamide gels¹; In situ hybridization on human chromosomes²

Properties

grade

for molecular biology

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)

30 mmHg ( 129 °C)

product line

BioUltra

assay

≥99.5% (T)

autoignition temp.

932 °F

total impurities

DNases, none detected

RNases, none detected

insoluble matter, passes filter test

phosphatases, none detected

proteases, none detected

ign. residue

≤0.1%

refractive index

n20/D 1.447(lit.)

n20/D 1.447

7-9 (25 °C, 1 M in H₂O)

210 °C(lit.)

2-3 °C(lit.)

solubility

H₂O: 10 M at 20 °C, clear, colorless

density

1.134 g/mL at 25 °C(lit.)

anion traces

chloride (Cl^-): ≤1 mg/kg

sulfate (SO₄^2-): ≤10 mg/kg

cation traces

Ag: ≤5 mg/kg

Al: ≤1 mg/kg

As: ≤0.1 mg/kg

Ba: ≤1 mg/kg

Bi: ≤1 mg/kg

Ca: ≤5 mg/kg

Cd: ≤1 mg/kg

Co: ≤1 mg/kg

Cr: ≤1 mg/kg

Cu: ≤1 mg/kg

Fe: ≤1 mg/kg

K: ≤20 mg/kg

Li: ≤1 mg/kg

Mg: ≤1 mg/kg

Mn: ≤1 mg/kg

Mo: ≤1 mg/kg

NH₄⁺: ≤1000 mg/kg

Na: ≤20 mg/kg

Ni: ≤1 mg/kg

Pb: ≤1 mg/kg

Sr: ≤1 mg/kg

Tl: ≤5 mg/kg

Zn: ≤1 mg/kg

10 M in H₂O

UV absorption

λ: 260 nm A_max: 0.4

λ: 280 nm A_max: 0.1

References

Cited Reference

1. R.C. Ogden, D.A. Adams Meth. Enzymol. 152, 67, (1987)

2. , G. Wilkinson, ed. In Situ Hybridization: A practical approach, 147, (1992)

Reference

, Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY , (1989), 9.47

, Ausubel, F.G. et al., ed Current Protocols in Molecular Biology New York , (1999), 2.10.11-2.10.12

Casey, J. and Davidson, N., Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide. Nucleic Acids Res. 4, 1539, (1977)

Merck

Merck 13,4261

Beilstein

Beil. 2,IV,45

Fieser

Fieser 1,402 / Fieser 2,201 / Fieser 3,147

reference

Aldrich MSDS 1, 979:B / Corp MSDS 1 (1), 1734:B / FT-IR 2 (1), 1254:A / FT-IR 1 (1), 747:A / IR-Spectra (3), 437:A / IR-Spectra (2), 387:A / NMR-Reference 2 (1), 629:A / RegBook 1 (1), 877:A / Sax 6, 1452 / Structure Index 1, 134:A:1 / Vapor Phase 3, 777:A

Safety

Hazard Codes

Risk Statements

Safety Statements

53-45

WGK Germany

RTECS

LQ0525000

UEL

19.00%

LEL

2.70%

Flash Point(F)

302 °F

Flash Point(C)

150 °C

Related Categories

... Denaturation > Acids and Bases

... Denaturation > Alphabetical

... Molecular Biology Reagents > BioUltra

... F > FM - FZ

... DNA & RNA Purification > Reagents

FT-IR Raman

47671 Data Sheet 59kb

MSDS

Certificate of Analysis

More Information