49419
Fluka

L-Glutamine

BioUltra, ≥99.5% (NT)

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Synonym

(S)-2,5-Diamino-5-oxopentanoic acid

L-Glutamic acid 5-amide

Molecular Formula

H₂NCOCH₂CH₂CH(NH₂)CO₂H

Molecular Weight

146.14

CAS Number

56-85-9

Beilstein Registry Number

1723797

MDL number

MFCD00008044

PubChem Substance ID

24872884

EG/EC Number

200-292-1

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Descriptions

Biochem/physiol Actions

L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Other Notes

In culture medium for hypothalamic neurons¹; Spacer in isotachophoresis of serum proteins²; Growth requirement of various microorganisms³.

Properties

product line

BioUltra

assay

≥99.5% (NT)

optical activity

[α]20/D +33.0±1°, c = 5% in 5 M HCl

total impurities

insoluble matter, passes filter test

≤0.5% foreign amino acids

ign. residue (900 °C)

≤0.1% (as SO₄)

loss

≤0.1% loss on drying, 110 °C

5.0-6.0 (25 °C, 0.1 M in H₂O)

185 °C (dec.)(lit.)

solubility

H₂O: 0.1 M at 20 °C, clear, colorless

anion traces

chloride (Cl^-): ≤50 mg/kg

sulfate (SO₄^2-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg

As: ≤0.1 mg/kg

Ba: ≤5 mg/kg

Bi: ≤5 mg/kg

Ca: ≤10 mg/kg

Cd: ≤5 mg/kg

Co: ≤5 mg/kg

Cr: ≤5 mg/kg

Cu: ≤5 mg/kg

Fe: ≤5 mg/kg

K: ≤50 mg/kg

Li: ≤5 mg/kg

Mg: ≤5 mg/kg

Mn: ≤5 mg/kg

Mo: ≤5 mg/kg

Na: ≤50 mg/kg

Ni: ≤5 mg/kg

Pb: ≤5 mg/kg

Sr: ≤5 mg/kg

Zn: ≤5 mg/kg

0.1 M in H₂O

UV absorption

λ: 260 nm A_max: 0.01

λ: 280 nm A_max: 0.01

References

Cited Reference

1. C. Loudes et al. Meth. Enzymol. 103, 313, (1983) abstract

2. T. Yagi et al., H. Hirai, ed. Electrophoresis ’83, ed. H. Hirai Berlin , 503, (1984)

3. CRC Handbook of Microbiology Cleveland, Ohio , (1974) 4, 1

Reference

McMahon, H.T., Nicholls, D.G. J. Neurochem. 54, 373, (1990)

Merck

Merck 13,4484

Beilstein

Beil. 4,491 / Beil. 4,IV,3038

reference

Corp MSDS 1 (1), 1777:C / FT-IR 2 (1), 1326:D / FT-IR 1 (1), 782:D / FT-NMR 1 (1), 1278:B / IR-Spectra (3), 463:D / IR-Spectra (2), 410:E / RegBook 1 (1), 915:O / Sigma FT-IR 1 (1), 175:A / Structure Index 1, 142:D:2

Safety

WGK Germany

RTECS

MA2275100

Related Categories

... Amino Acids and Peptides > Amino Acids

... Biochemicals Found in Plants > Amino acids

... Amino Acids and Peptides > BioUltra

FT-IR Condensed Phase

FT-IR Raman

FT-NMR

MSDS

Certificate of Analysis

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