49767
Fluka

Glycerol

BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)

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Synonym

1,2,3-Propanetriol

Glycerin

Molecular Formula

HOCH₂CH(OH)CH₂OH

Molecular Weight

92.09

CAS Number

56-81-5

Beilstein Registry Number

635685

MDL number

MFCD00004722

PubChem Substance ID

24873136

EG/EC Number

200-289-5

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Descriptions

Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Other Notes

Component of loading buffer in agarose gel electrophoresis of nucleic acids previously denatured with glyoxal¹; Preparation of phage and plasmid DNA, for the storage of pure cultures²

Properties

grade

for molecular biology

vapor density

3.1 (vs air)

vapor pressure

<1 mmHg ( 20 °C)

product line

BioUltra

assay

≥99.5% (GC)

autoignition temp.

698 °F

total impurities

DNases, none detected

RNases, none detected

insoluble matter, passes filter test

phosphatases, none detected

proteases, none detected

ign. residue

≤0.1%

refractive index

n20/D 1.474(lit.)

n20/D 1.474

5.5-8 (25 °C, 5 M in H₂O)

182 °C/20 mmHg(lit.)

20 °C(lit.)

solubility

H₂O: 5 M at 20 °C, clear, colorless

density

1.25 g/mL(lit.)

anion traces

chloride (Cl^-): ≤1 mg/kg

sulfate (SO₄^2-): ≤10 mg/kg

cation traces

Ag: ≤5 mg/kg

Al: ≤1 mg/kg

As: ≤0.1 mg/kg

Ba: ≤1 mg/kg

Bi: ≤1 mg/kg

Ca: ≤5 mg/kg

Cd: ≤1 mg/kg

Co: ≤1 mg/kg

Cr: ≤1 mg/kg

Cu: ≤1 mg/kg

Fe: ≤1 mg/kg

K: ≤20 mg/kg

Li: ≤1 mg/kg

Mg: ≤1 mg/kg

Mn: ≤1 mg/kg

Mo: ≤1 mg/kg

NH₄⁺: ≤5 mg/kg

Na: ≤20 mg/kg

Ni: ≤1 mg/kg

Pb: ≤1 mg/kg

Sr: ≤1 mg/kg

Tl: ≤5 mg/kg

Zn: ≤1 mg/kg

5 M in H₂O

UV absorption

λ: 260 nm A_max: 0.05

λ: 280 nm A_max: 0.04

References

Cited Reference

1. R.C. Ogden, D.A. Adam Meth. Enzymol. 152, 79, (1987)

2. H. Miller Meth. Enzymol. 152, 145, (1987) abstract

Reference

, Andrews, A.T. Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications 2nd ed., New York , (1988), 252

, Link, A.J. ed., 2-D Proteome Analysis Protocols. Methods Mol. Biol. Totowa, NJ , (1999) 112

Merck

Merck 13,4497

Beilstein

Beil. 1,IV,2751

reference

Aldrich MSDS 1, 993:A / Corp MSDS 1 (1), 1782:D / FT-IR 2 (1), 265:B / FT-IR 1 (1), 183:A / FT-NMR 1 (1), 283:C / IR-Spectra (3), 112:A / IR-Spectra (2), 103:G / NMR-Reference 2 (1), 171:B / RegBook 1 (1), 185:A / Sax 6, 1479 / Sigma FT-IR 1 (1), 1340:B / Structure Index 1, 24:C:7 / Vapor Phase 3, 262:A

Safety

WGK Germany

RTECS

MA8050000

LEL

0.90%

Flash Point(F)

350.6 °F

Flash Point(C)

177 °C