50933
Fluka

Guanidine hydrochloride

BioUltra, for molecular biology, ≥99.5% (AT)

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Synonym

Aminoformamidine hydrochloride

Aminomethanamidine hydrochloride

Guanidinium chloride

Molecular Formula

NH₂C(=NH)NH₂ · HCl

Molecular Weight

95.53

CAS Number

50-01-1

Beilstein Registry Number

3591990

MDL number

MFCD00013026

PubChem Substance ID

24873453

EG/EC Number

200-002-3

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Descriptions

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Other Notes

Denaturant. Isolation of RNA¹

Properties

grade

for molecular biology

product line

BioUltra

assay

≥99.5% (AT)

total impurities

DNases, none detected

RNases, none detected

insoluble matter, passes filter test

phosphatases, none detected

proteases, none detected

ign. residue

≤0.05% (as SO₄)

loss

≤0.05% loss on drying, 20 °C (HV)

4.5-6.0 (25 °C, 6 M in H₂O)

180-185 °C(lit.)

solubility

H₂O: 6 M at 20 °C, clear, colorless

anion traces

sulfate (SO₄^2-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg

As: ≤0.1 mg/kg

Ba: ≤5 mg/kg

Bi: ≤5 mg/kg

Ca: ≤10 mg/kg

Cd: ≤5 mg/kg

Co: ≤5 mg/kg

Cr: ≤5 mg/kg

Cu: ≤5 mg/kg

Fe: ≤5 mg/kg

K: ≤50 mg/kg

Li: ≤5 mg/kg

Mg: ≤5 mg/kg

Mn: ≤5 mg/kg

Mo: ≤5 mg/kg

Na: ≤50 mg/kg

Ni: ≤5 mg/kg

Pb: ≤5 mg/kg

Sr: ≤5 mg/kg

Zn: ≤5 mg/kg

6 M in H₂O

UV absorption

λ: 260 nm A_max: 0.03

λ: 280 nm A_max: 0.02

References

Cited Reference

1. R.J. MacDonald et al. Meth. Enzymol. 152, 219, (1987) abstract

Reference

Rudloph, R. and Lilie, H., In vitro folding of inclusion body proteins. FASEB J. 10, 49-56, (1996) abstract

Naglak, T.J. and Wang, H.Y., Recovery of a foreign protein from the periplasm of Escherichia coli by chemical permeabilization. Enzyme Microb. Technol. 12, 603, (1990)

Marston, F.A.O. and Hartley, D.L., Solubilization of protein aggregates. Meth. Enzymol. 182, 264-276, (1990)

Mukhopadhyay, A., Inclusion bodies and purification of proteins in biologically active forms. Adv. Biochem. Eng. Biotechnol. 56, 61-109., (1997) abstract

Levine, A.D., et al., High Level Expression and Refolding of Mouse Interleukin 4 Synthesized in Escherichia coli. J. Biol. Chem. 270, 7445-7452, (1995)

Merck

Merck 13,4578

Beilstein

Beil. 3,IV,150

reference

Aldrich MSDS 1, 998:C / Corp MSDS 1 (1), 1803:D / FT-IR 2 (1), 1388:B / FT-IR 1 (1), 818:A / IR-Spectra (3), 485:D / IR-Spectra (2), 429:D / RegBook 1 (1), 961:K / Sax 6, 1488 / Sigma FT-IR 1 (2), 597:D / Sigma FT-IR 1 (2), 597:C / Structure Index 1, 149:C:7

Safety

Hazard Codes

Risk Statements

22-36/38

Safety Statements

WGK Germany

RTECS

MF4300000

3-10