56745
Fluka

Imidazole

BioUltra, for luminescence, ≥99.5% (GC)

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Synonym

1,3-Diaza-2,4-cyclopentadiene

Glyoxaline

Molecular Formula

C₃H₄N₂

Molecular Weight

68.08

CAS Number

288-32-4

Beilstein Registry Number

103853

MDL number

MFCD00005183

EG/EC Number

206-019-2

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Descriptions

Application

Excellent for buffers in the range of pH 6.2-7.8

Other Notes

Kinetic investigations with pig kidney Na⁺,K⁺-ATPase, by stopped-flow fluorimetry^1,²

Properties

grade

for luminescence

vapor pressure

<1 mmHg ( 20 °C)

product line

BioUltra

assay

≥99.5% (GC)

total impurities

insoluble matter, passes filter test

≤0.2% water

ign. residue (900 °C)

≤0.05%

fluorescence

λ_ex 200-600 nm; λ_em 300-800 nm (corresponds)

9.5-11.0 (25 °C, 0.1 M in H₂O)

pKa (25 °C)

6.95

256 °C(lit.)

88-91 °C(lit.)

solubility

H₂O: 0.1 M at 20 °C, clear, colorless

anion traces

chloride (Cl^-): ≤500 mg/kg

sulfate (SO₄^2-): ≤50 mg/kg

cation traces

Al: ≤10 mg/kg

As: ≤0.1 mg/kg

Ba: ≤5 mg/kg

Bi: ≤5 mg/kg

Ca: ≤50 mg/kg

Cd: ≤5 mg/kg

Co: ≤5 mg/kg

Cr: ≤5 mg/kg

Cu: ≤5 mg/kg

Fe: ≤5 mg/kg

K: ≤50 mg/kg

Li: ≤5 mg/kg

Mg: ≤5 mg/kg

Mn: ≤5 mg/kg

Mo: ≤5 mg/kg

Na: ≤50 mg/kg

Ni: ≤5 mg/kg

Pb: ≤5 mg/kg

Sr: ≤5 mg/kg

Zn: ≤5 mg/kg

0.1 M in H₂O

UV absorption

λ: 260 nm A_max: 0.10

λ: 280 nm A_max: 0.10

References

Cited Reference

1. D. J. Kane et al. Biochemistry 37, 4581, (1998) abstract

2. D. J. Kane et al. Biochemistry 36, 13406, (1997) abstract

Reference

R. Breslow, Review: As RNase model. Acc. Chem. Res. 24, 317, (1991)

B. Storrie, E.A. Madden, Buffer for assay of horseradish peroxidase. Meth. Enzymol. 182, 217, (1990)

Merck

Merck 13,4935

Beilstein

Beil. 23,V,4,191

Fieser

Fieser 1,492 / Fieser 2,220

reference

Aldrich MSDS 1, 1083:B / Corp MSDS 1 (1), 1973:D / FT-IR 2 (3), 3503:B / FT-IR 1 (2), 611:D / FT-NMR 1 (3), 75:B / IR-Spectra (3), 1217:G / IR-Spectra (2), 1060:E / NMR-Reference 2 (2), 484:D / RegBook 1 (2), 2351:A / Sax 6, 1605 / Sigma FT-IR 1 (2), 648:C / Structure Index 1, 371:D:6 / Vapor Phase 3, 1483:C

Safety

Hazard Codes

Risk Statements

22-34

Safety Statements

26-36/37/39-45

RIDADR

UN2923 8/PG 3

WGK Germany

RTECS

NI3325000

Flash Point(F)