Biochem/physiol Actions
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Other Notes
Substrate specificity: preferentially hydrolyzes the 1- and 3-position (medium to long fatty acids); optimum pH 5.5-8.5; optimum temperature 30-45°C; Purification and properties1; Hydrolyzes the dibutylester of bishydroxymethyl-1,7-dioxaspiroundecane2
Unit Definition
1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from triglycerides per minute at pH 8.0 and 37 °C (olive oil as substrate]; 2000 U as described above (using olive oil) are equivalent to ~1 U using triolein, Fluka No. 62314, as substrate.
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