Luciferase from Vibrio fischeri (Photobacterium f) 

Partially purified, soluble extracts containing FMN-dependent luciferase and NADH- and NADPH-dependent FMN reductases. Produces light in a system containing FMN, NADH or NADPH, and n-decyl aldehyde.

Produces light in a system with FMN, NADH and an aliphatic aldehyde containing more than 8-C atoms.; The pack size is sufficient for more than 200 determinations of NADH in a concentration range of 10^-10 mol/l.; A solution in water (5 mg lyophilisate/0.5 ml) is stable for at least one week and deep-frozen for several months.; The biochemistry and molecular biology of bacterial bioluminescence^1,2

BioChemika

~8% protein basis

powder

lyophilized

M_r ~79000

−20°C

1. T.O. Baldwin and M.M. Ziegler, F. Mueller, ed. Chem. Biochem. Flavoenzymes Boca Raton, Fl. 3, 467, (1992)

2. K. Green et al. Talanta 31, 173, (1984)

3