63690
Fluka

2-Mercaptoethanol

BioChemika, ≥99.0% (GC)

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Comparable product

M7154, 2-Mercaptoethanol

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Synonym

β-Mercaptoethanol

2-Hydroxyethylmercaptan

BME

Thioethylene glycol

Molecular Formula

HSCH₂CH₂OH

Molecular Weight

78.13

CAS Number

60-24-2

Beilstein Registry Number

773648

MDL number

MFCD00004890

EG/EC Number

200-464-6

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Descriptions

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

A reducing agent in fluorescent reaction of o-phthaldialdehyde and amino acids in alkaline medium^1,^2,³; For the dissociation of proteins^4,⁵

Properties

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

product line

BioChemika

assay

≥99.0% (GC)

expl. lim.

18 %

concentration

14.3 M (pure liquid)

ign. residue

≤0.01%

refractive index

n20/D 1.500(lit.)

n20/D 1.501

157 °C(lit.)

density

1.114 g/mL at 25 °C(lit.)

cation traces

Al: ≤0.5 mg/kg

Ba: ≤0.1 mg/kg

Bi: ≤0.1 mg/kg

Ca: ≤0.5 mg/kg

Cd: ≤0.05 mg/kg

Co: ≤0.02 mg/kg

Cr: ≤0.02 mg/kg

Cu: ≤0.02 mg/kg

Fe: ≤0.1 mg/kg

K: ≤0.5 mg/kg

Li: ≤0.1 mg/kg

Mg: ≤0.1 mg/kg

Mn: ≤0.02 mg/kg

Mo: ≤0.1 mg/kg

Na: ≤1 mg/kg

Ni: ≤0.05 mg/kg

Pb: ≤0.1 mg/kg

Sr: ≤0.1 mg/kg

Zn: ≤0.1 mg/kg

storage temp.

2-8°C

References

Cited Reference

1. M. Roth Anal. Chem. 43, 880, (1971) abstract

2. R.F. Chen et al. Biochim. Biophys. Acta 576, 440, (1979) abstract

3. C.C. Goodon et al. Anal. Biochem. 115, 203, (1981) abstract

4. J. Barshard Science 131, 988, (1966)

5. H.T. Keutmann, J.T. Potts Anal. Biochem. 29, 175, (1966)

Merck

Merck 13,5894

Beilstein

Beil. 1,IV,2428

reference

Aldrich MSDS 1, 1162:C / Corp MSDS 1 (2), 2209:A / FT-IR 2 (1), 415:B / FT-IR 1 (1), 262:B / FT-NMR 1 (1), 422:B / IR-Spectra (3), 154:F / IR-Spectra (2), 138:E / NMR-Reference 2 (1), 228:B / RegBook 1 (1), 281:L / Sax 6, 1744 / Sigma FT-IR 1 (2), 700:C / Structure Index 1, 38:D:7 / Vapor Phase 3, 344:C

Safety

Hazard Codes

T,N

Risk Statements

20/22-24-34-51/53

Safety Statements

26-36/37/39-45-61

RIDADR

UN2966 6.1/PG 2

WGK Germany

RTECS

KL5600000