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75403 
Fluka 

Crystallization Cryo Kit for Proteins 

BioChemika  
Synonym 
Protein Cryo Crystallography Kit

 
 
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Price and Availability
Descriptions
Features and Benefits  
• Comprehensive, efficient screening
• Stringent purity standards
• Prefabricated reagents, flow charts, and observation sheets
• Low incidence of undesirable solubility effects

 
General description  
The Protein Cryo Crystallography Kit provides a rapid, empirical, and efficient screening method to determine the solubility and optimal starting conditions for the crystallization of biological macromolecules in the presence of a cryoprotectant (primarily glycerol). Based on known or published data from various proteins, the Cryo Kit uses a sparse matrix method and minimal amount of protein sample to explore a broad range of buffers, pH, and precipitants. The screening range and probing ability of the Cryo Kit are comparable to those of the Basic Kit. A cryoprotectant is added to freeze crystals in an amorphous glass at -173°C.
The kit contains 50 components (10 mL per vial).
 
  
The Protein Cryo Kit is based on the "salting out" principal: increasing the concentration of a precipitating reagent and cryoprotectant depletes the macromolecules of bound water and leads to their precipitation or crystallization1.
 
Other Notes  
Each Component is available as 100 mL package. Inquire for other package sizes.
 
Properties
product line 
BioChemika
 
storage temp. 
2-8°C
References
Cited Reference 
1. E.F. Garman, E.P. Mitchell J. Appl. Cryst. 29, 584, (1996)
Components
Kit component also available separately 
Imidazole pH 6.5; 0.07 M - Sodium acetate 0.7 M - Glycerol 30% solution, BioChemika 71591
 
 
Sodium citrate tribasic pH 5.6; 0.07 M – Ammonium phosphate monobasic 0.7 M – Glycerol 30% (v/v) solution, BioChemika 72871
 
 
Magnesium chloride 0.18 M – HEPES-Na pH 7.5; 0.09M – 2-Propanol 27% (w/v) – Glycerol 10% solution, BioChemika 72872
 
 
Sodium acetate 0.17 M – TRIS hydrochloride pH 8.5; 0.085 M – Polyethylene glycol 4000 25.5% (w/v) – Glycerol 15% (v/v) solution, BioChemika 73803
 
 
Ammonium acetate 0.16M – TRIS hydrochloride pH 8.5; 0.08M – 2-Propanol 24% (v/v) – Glycerol 20% (v/v) solution, BioChemika 74613
 
 
Sodium citrate tribasic 0.14M – Sodium cacodylate pH 6.5; 0.07M – 2-Propanol 21% (v/v) – Glycerol 30% (v/v) solution, BioChemika 74614
 
 
Magnesium chloride 0.2M – HEPES Na pH 7.5; 0.1M – Polyethylene glycol 400 30% (w/v) solution, BioChemika 76525
 
 
Ammonium acetate 0.2M – Sodium citrate tribasic pH 5.6; 0.1M – 2-Methyl-2,4-pentanediol 30% (w/v) solution, BioChemika 76658
 
 
HEPES Na pH 7.5; 0.065M – Potassium sodium tartrate 0.52M – Glycerol 35% (v/v) solution, BioChemika 76659
 
 
Ammonium acetate 0.17M – Sodium citrate tribasic pH 5.6; 0.085M – Polyethylene glycol 4000 25.5% (w/v) – Glycerol 15% (v/v) solution, BioChemika 77437
 
 
Ammonium acetate 0.17M – Sodium acetate pH 4.6; 0.085M – Polyethylene glycol 4000 25.5% (w/v) – Glycerol 15% (v/v) solution, BioChemika 77582
 
 
Magnesium acetate 0.2M – Sodium cacodylate pH 6.5; 0.1M – 2-Methyl-2,4-pentanediol 30% (v/v) solution 79974
 
 
Sodium citrate tribasic 0.2M – TRIS hydrochloride pH 8.5; 0.1M – Polyethylene glycol 400 30% (w/v) solution 80055
 
 
Ammonium sulfate 0.17M – Sodium cacodylate pH 6.5; 0.085M – Polyethylene glycol 8000 25.5% (w/v) – Glycerol 15% (v/v) solution 80074
 
 
Lithium sulfate 0.17 M – TRIS hydrochloride pH 8.5; 0.085M – Polyethylen glycol 4000 25.5% (w/v) – Glycerol 15% (v/v) solution 80091
 
 
Magnesium acetate 0.16 M – Sodium cacodylate pH 6.5; 0.08M – Polyethylene glycol 8000 16% (w/v) – Glycerol 20% solution 80093
 
 
Sodium citrate tribasic 0.14M, HEPES Na pH 7.5; 0.07M, 2-Propanol 14% (v/v), Glycerol 30% solution 80384
 
 
Sodium acetate 0.17M – Sodium cacodylate pH 6.5; 0.085M – Polyethylene glycol 8000 25.5% (w/v) – Glycerol 15% (v/v) solution 80387
 
 
Calcium chloride 0.19 M – HEPES-Na pH 7.5; 0.095M – Polyethylene glycol 400; 26.6% (w/v) – Glycerol 5% solution 80556
 
 
Ammonium sulfate 0.17 M – Polyethylene glycol 8000 25.5% (w/v) – Glycerol 15% solution 80803
 
 
HEPES-Na pH 7.5; 0.085M – Polyethylene glycol 400 1.7% (v/v) – Ammonium sulfate 1.7M – Glycerol 15% (v/v) solution 80864
 
 
HEPES-Na pH 7.5; 0.075M – Sodium phosphate monobasic 0.6M – Potassium phosphate monobasic 0.6M – Glycerol 25% (v/v) solution 80924
 
 
Lithium sulfate 0.8 M – Polyethylene glycol 8000 1.6% (w/v) – Glycerol 20% (v/v) solution 81696
 
 
Lithium sulfate 0.4 M – Polyethylene glycol 8000 12% (w/v) – Glycerol 20% (v/v) solution 81713
 
 
Sodium formate 3.6 M – Glycerol 10% solution 81892
 
 
Sodium acetate pH 4.6; 0.07M – 1.4 M Sodium formate – Glycerol 30% (v/v) solution 81893
 
 
Sodium acetate pH 4.6; 0.07M – Polyethylene glycol 4000 5.6% (w/v) – Glycerol 30% solution 82216
 
 
HEPES-Na pH 7.5; 0.085M – 2-Propanol 8.5% (v/v) – Polyethylene glycol 4000 17% (w/v) – Glycerol 15% (v/v) solution 82809
 
 
Sodium acetate pH 4.6; 0.08 M – Ammonium sulfate 1.6 M – Glycerol 20% (v/v) solution 83196
 
 
Zinc acetate 0.16M – Sodium cacodylate pH 6.5; 0.08M – Polyethylene glycol 8000 14.4% (w/v) – Glycerol 20% (v/v) 83198
 
 
Potassium phosphate monobasic 0.04M – Polyethylene glycol 8000 16% (w/v) – Glycerol 20% (v/v) solution 83617
 
 
Calcium acetate 0.16 M – Sodium cacodylate pH 6.5; 0.08M – Polyethylene glycol 8000 14.4% (w/v) – Glycerol 20% 84461
 
 
TRIS hydrochloride pH 8.5; 0.08 M – Ammonium phosphate monobasic 1.6 M – Glycerol 20% (v/v) solution
 
 
Ammonium sulfate 0.17 M – Polyethylene glycol 4000 25.5% (w/v) – Glycerol 15% solution 87429
 
 
Ammonium sulfate 1.5 M – Glycerol 25% solution 89252
 
 
TRIS hydrochloride pH 8.5; 0.065M – Polyethylene glycol 8000 5.2% (w/v) – Glycerol 35% (v/v) solution 89725
 
 
HEPES-Na pH 7.5; 0.09M – Sodium citrate tribasic 1.26M – Glycerol 10% solution 89734
 
 
Polyethylene glycol 1500 24% (w/v) – Glycerol 20% (v/v) solution 90213
 
 
Magnesium formate 0.1M – Glycerol 50% (v/v) solution 91638
 
 
Sodium citrate tribasic pH 5.6; 0.095M – 2-Propanol 19% (v/v) – Polyethylene glycol 4000 19% (w/v) – Glycerol 5% solution 91959
 
 
Calcium chloride 0.02 M – Sodium acetate 0.1M – 2-Methyl-2,4-pentanediol 30% (v/v) solution 92599
 
 
Magnesium chloride 0.16M – TRIS hydrochloride pH 8.5; 0.08M – Polyethylene glycol 4000 24% (w/v) – Glycerol 20% (v/v) solution 93189
 
 
Sodium citrate tribasic 0.2M – HEPES-Na pH 7.5; 0.1M – 2-Methyl-2,4-pentanediol 30% (v/v) solution 93949
 
 
TRIS hydrochloride pH 8.5; 0.075 M – Ammonium sulfate 1.5 M – Glycerol 25% (v/v) solution 94030
 
 
Ammonium phosphate monobasic 0.26 M – Glycerol 35% (v/v) solution 96347
 
 
Potassium sodium tartrate 0.26 M – Glycerol 35% (v/v) solution 96864
 
 
Calcium chloride 0.14M – Sodium acetate pH 4.6; 0.07M – 2-Propanol 14% (v/v) – Glycerol 30% (v/v) solution, BioChemika 73806
 
 
Sodium cacodylate pH 6.5; 0.07M – Sodium acetate 0.98M – Glycerol 30% (v/v) solution 79973
 
 
Ammonium sulfate 0.16 M – Sodium acetate pH 4.6; 0.08M – Polyethylene glycol 4000 20% (w/v) – Glycerol 20% (v/v) solution 80053
 
 
HEPES Na pH 7.5; 0.075M – Lithium sulfate 1.125M – Glycerol 25% (v/v) solution 80084
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