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80701 
Fluka 

Crystallization Kit for DNA 

BioChemika  
Synonym 
DNA Crystallization Kit

 
 
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Price and Availability
Descriptions
Features and Benefits  
• Comprehensive, efficient screening
• Extremely pure, sterile reagents
• Prefabricated reagents and observation sheets
• Low incidence of undesirable solubility effects

 
General description  
The Crystallization Kit for DNA is an empirical screening method for the direct determination of suitable crystallization conditions for nucleic acids.

The kit is developed according to the described method of Nowakowski et al.1, Baeyens, Jancarik and Holbrook2 These ready-made solutions represent a screen of conditions, from which many were observed to produce crystals from DNA oligomers and transfer RNA. This screen provides high efficiency in crystallization of RNA oligomers. Thus it provides a completion of our crystallization kit for DNA.

The Crystallization Kit for DNA consists of 48 different formulations covering a range of different buffers (pH 6.0-8.0), different types of precipitants (esp. PEG 400 and MPD), and inorganic salts including magnesium salts. All solutions are sterile filtered using 0.22 micron filters. The kit contains 10 ml of each formulation, but all solutions are available separately as 100 ml bottles. Larger quantities are available on request.
 
Other Notes  
Every component can be ordered separately in pack size of 100 mL, but inquiries for different sizes are also welcome.
 
  
The Kit contains 48 components with 10 mL each.
 
Properties
product line 
BioChemika
 
mfr. no. 
Fluka
 
storage temp. 
2-8°C
References
Cited Reference 
1. Nowakowski, J., et al. Acta Crystallogr. D (Biol. Crystallogr.) 55, 1885-1892, (1999) abstract
 
 
2. Baeyens,K.J., et al. Acta Crystallogr. D (Biol. Crystallogr.) 50, 764-767, (1994) abstract
Safety
Hazard Codes 

 
Risk Statements 

 
Safety Statements 

 
RIDADR 
UN 3316 6.1/PG 3
Components
Kit component also available separately 
HEPES pH 7.5; 0.05 M − Magnesium chloride 80 mM − Spermine 2.5 mM solution, BioChemika 28674
 
 
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 18 mM − Spermine 2.25 mM − Copper sulfate 1 mM − Isopropanol 9% solution, BioChemika 76475
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 18 mM − Spermine 0.9 mM − Cobalt(III)-hexamine 1.8 mM − Isopropanol 9% solution, BioChemika 42393
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 18 mM − Spermine 2.25 mM − Isopropanol 9% solution, BioChemika 89253
 
 
Cacodylate pH 7.0; 0.05 M − Magnesium chloride 18 mM − Spermine 2.25 mM − Cobalt(III)-hexamine 0.9 mM − 2-Methyl-2,4-pentanediol 4.5% solution, BioChemika 67516
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 36mM − Spermine 2.25 mM − PEG 400 5% solution, BioChemika 90746
 
 
Sodium succinate pH 5.5; 0.05 M − Magnesium chloride 10 mM − Cobalt(III)-hexamine 2.0 mM − Isopropanol 10% solution, BioChemika 89264
 
 
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 20 mM − Spermine 1.0 mM − Ethanol 15% solution, BioChemika 90971
 
 
Cacodylate pH 7.0; 0.05 M − Magnesium chloride 20 mM − Spermine 1.0 mM − Cobalt(III)-hexamine 1.0 mM − Ethanol 15% solution, BioChemika 51189
 
 
Cacodylate pH 7.0; 0.05 M − Magnesium chloride 5 mM − Spermidine 1.0 mM − tert-Butanol 10% solution, BioChemika 19693
 
 
Cacodylate pH 7.0; 0.05 M − Magnesium chloride 30 mM − Spermine 2.5 mM − PEG 400 5% solution, BioChemika 93084
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 100 mM − Cobalt(III)-hexamine 2.0 mM − Isopropanol 5% solution, BioChemika 30517
 
 
TRIS pH 8.0; 0.05 M − Magnesium chloride 10 mM − Cobalt(III)-hexamine 1.0 mM − Ethanol 20% solution, BioChemika 51804
 
 
HEPES pH 7.5; 0.05 M − Magnesium chloride 20 mM − Spermine 1.0 mM − PEG 8000 5% solution, BioChemika 90748
 
 
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 20 mM − Spermine 2.5 mM − PEG 4000 5% solution, BioChemika 95109
 
 
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 10 mM − Spermine 2.5 mM − Calcium chloride 5 mM − Isopropanol 10% solution, BioChemika 89263
 
 
Cacodylate pH 7.0; 0.05 M − Magnesium chloride 9 mM − Spermine 2.25 mM − Cobalt(III)-hexamine 1.8 mM − Spermidine 0.9 mM − PEG 400 5% solution, BioChemika 89269
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 10 mM − Spermine 2.5 mM − Copper sulfate 1 mM − Isopropanol 10% solution, BioChemika 56961
 
 
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 20 mM − Spermine 1.0 mM − Calcium chloride 2 mM − 1,5 Hexanediol 10% solution, BioChemika 91373
 
 
HEPES pH 7.5; 0.05 M − Magnesium chloride 15 mM − Spermidine 1.0 mM − Dioxan 10% solution, BioChemika 30193
 
 
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 15 mM − Spermine 3.0 mM − PEG 400 10% solution, BioChemika 67198
 
 
Cacodylate pH 6.5; 0.05 M − Spermine 2.5 mM − Calcium chloride 18 mM − 2-Propanol 9% solution, BioChemika 97863
 
 
Cacodylate pH 6.5; 0.05 M − Spermine 2.0 mM − Cobalt(III)-hexamine 1.0 mM − Calcium chloride 80 mM solution, BioChemika 55784
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 5 mM − Cobalt(III)-hexamine 2.5 mM solution, BioChemika 07312
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 30 mM − Spermine 1.0 mM − Lithium sulfate 1.3 M solution, BioChemika 19485
 
 
Cacodylate pH 6.0; 0.05 M − Calcium acetate 200 mM − Isopropanol 5% solution, BioChemika 68446
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 100 mM − Cobalt(III)-hexamine 1.0 mM − Ethanol 10% solution, BioChemika 80215
 
 
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 10 mM − Spermidine 2.5 mM − Sodium chloride 2.5 M solution, BioChemika 19348
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 10 mM − Sodium citrate 200 mM − Isopropanol 5% solution, BioChemika 16829
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 15 mM − Spermine 10.0 mM − Lithium sulfate 2.0 M solution, BioChemika 44046
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 20 mM − Spermine 1.0 mM − Ammonium sulfate 2.0 M solution, BioChemika 73519
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 10 mM − Spermine 1.5 mM − Ammonium sulfate 3.0 solution, BioChemika 79853
 
 
HEPES pH 7.5; 0.05 M − Magnesium chloride 15 mM − Spermine 1.0 mM − Ammonium sulfate 1.0 M solution, BioChemika 66017
 
 
Cacodylate pH 6.0; 0.05 M − Calcium acetate 200 mM − Sodium chloride 2.5 M solution, BioChemika 18154
 
 
Cacodylate pH 6.0; 0.05 M − Calcium acetate 200 mM − Cobalt(III)-hexamine 1.0 mM − Lithium chloride 2.0 M solution, BioChemika 42794
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 15 mM − Spermidine 5.0 mM − Cobalt(III)-hexamine 1.0 mM − Sodium chloride 2.0 M solution, BioChemika 40377
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 200 mM − Sodium chloride 100 mM − PEG 1000 20% solution, BioChemika 15873
 
 
TRIS pH 7.5; 0.05 M − Magnesium chloride 50 mM − Sodium tartrate 1.0 M solution, BioChemika 42029
 
 
TRIS pH 7.5; 0.05 M − Magnesium chloride 200 mM − Sodium chloride 2.5 M solution, BioChemika 44259
 
 
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 200 mM − Potassium chloride 2.5 M solution, BioChemika 41172
 
 
TRIS pH 8.0; 0.05 M – Magnesium chloride 200 mM – Ethanol 15% solution, BioChemika 75771
 
 
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 15 mM − Spermidine 5.0 mM − Lithium sulfate 2.0 M solution, BioChemika 92213
 
 
Cacodylate pH 6.0; 0.05 M − Magnesium acetate 20 mM − Spermine 0.5 mM − Sodium chloride 100 mM − 2-Methyl-2,4-pentanediol 25% solution, BioChemika 53245
 
 
Sodium succinate 0.05 M − Magnesium chloride 20 mM − Spermine 0.5 mM − Ammonium sulfate 3.0 solution, BioChemika 43374
 
 
Cacodylate pH 6.5; 0.05 M − Cobalt(III)-hexamine 5 mM − Potassium chloride 2.5 M solution, BioChemika 44401
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 50 mM − Cobalt(III)-hexamine 2.0 mM − Lithium sulfate 1.5 M solution, BioChemika 05556
 
 
Cacodylate pH 6.5; 0.05 M − Spermine 1.0 mM − Cobalt(III)-hexamine 2.0 mM − Calcium chloride 30 mM − Lithium chloride 2.0 M solution, BioChemika 04359
 
 
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 10 mM − Spermine 50 mM solution, BioChemika 66953
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